|本期目录/Table of Contents|

[1]马 倩,王 可,王曙东,等.OBC/SF复合软骨支架的制备及性能[J].丝绸,2017,54(10):101104.[doi:10.3969/j.issn.1001-7003.2017.10.004]
 MA Qian,WANG Ke,WANG Shudong,et al.Preparation and performance of oxidized bacterial cellulose/silk fibroin composite cartilage scaffold[J].Journal of Silk,2017,54(10):101104.[doi:10.3969/j.issn.1001-7003.2017.10.004]
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OBC/SF复合软骨支架的制备及性能(PDF)
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《丝绸》[ISSN:1001-7003/CN:33-1122/TS]

卷:
54
期数:
2017年10期
页码:
101104
栏目:
研究与技术
出版日期:
2017-10-20

文章信息/Info

Title:
Preparation and performance of oxidized bacterial cellulose/silk fibroin composite cartilage scaffold
文章编号:
1001-7003(2017)10-0018-06
作者:
马 倩王 可王曙东朱立成马丕波朱玲玲
1. 盐城工业职业技术学院 纺织服装学院,江苏 盐城 224005;2. 武汉纺织大学 湖北省纺织新材料及其应用重点实验室, 武汉 430200;3.江南大学 生态纺织教育部重点实验室,江苏 无锡 214122
Author(s):
MA Qian WANG Ke WANG Shudong ZHU Licheng MA Pibo ZHU Lingling
 1.College of Textile and Clothing, Yancheng Vocational Institute of Industry Technology, Yancheng 224005, China; 2. Hubei New Textile Material & Application Key Laboratory, Wuhan Textile University, Wuhan 430200, China; 3. Ministry of Educations Key Laboratory of Eco-textiles, Jiangnan University, Wuxi 214112, China
关键词:
BMP-2细菌纤维素氧化丝蛋白水凝胶电凝胶技术
Keywords:
BMP-2 oxidation bacterial cellulose silk fibroin hydrogel gel electrophoresis
分类号:
T340.64
doi:
10.3969/j.issn.1001-7003.2017.10.004
文献标志码:
A
摘要:
采用TEMPO/NaBr/NaClO体系对细菌纤维素(BC)进行氧化改性,然后以氧化细菌纤维素(OBC)、丝蛋白(SF)作为基础材料,负载骨形态发生蛋白(BMP-2),经电凝胶技术制备负载BMP-2的OBC/SF复合水凝胶。通过扫描电镜(SEM)、孔隙率、力学性能、体外降解率测试对所制备的复合水凝胶进行表征,将软骨细胞种植于负载BMP-2的OBC/SF复合水凝胶支架上进行体外联合培养,观察软骨细胞在支架中的增殖情况。结果表明该OBC/SF复合水凝胶是一种具有细腻、疏松的双网络孔隙结构,良好的降解性能、优异的力学性能,适合细胞增殖的骨组织工程支架材料BMP-2的加入有诱导软骨细胞分化,维持软骨细胞特点的作用。
Abstract:
The bacterial cellulose was modified by TEMPO/NaBr/NaClO-mediated oxidation. The oxidized bacterial cellulose/silk fibroin (OBC/SF) composite hydrogel containing bone morphogenic protein (BMP-2) for cartilage repair was prepared by gel electrophoresis. The prepared composite hydrogel was characterized by Scanning Electron Microscopy (SEM), porosity, mechanical testing and in-vitro degradation. The chondrocytes were grown on the prepared OBC/SF composite hydrogel scaffold for in-vitro culture. The growth of the chondrocytes on the composite scaffold was observed. Results show that the prepared OBC/SF composite hydrogel is an ideal scaffold for bone tissue engineering, with fine and loose double network structure, appropriate degradability, excellent mechanical properties and native environment for cell proliferation. Besides, the addition of BMP-2 contributes to the differentiation of chondrocytes and helps to maintain the characteristics of chondrocytes

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备注/Memo

备注/Memo:
基金项目:江苏省高校自然科学基金项目(16KJB540005);湖北省纺织新材料及其应用重点实验室开放课题(Fzxcl2017016);生态纺织教育部重点实验室(江南大学)开放课题(KLET1601);江苏省盐城市农业科技指导性计划项目(YKN2016012、YKN2016013) ;江苏高校品牌专业建设工程资助项目(PPZY2015C254);盐城工业职业技术学院院级重点课题(ygy201501);江苏省高校优秀中青年教师境外研修计划项目(苏教师2014〕24号);江苏省高等学校大学生创新创业训练计划项目(201713752017X)
收稿日期: 2016-12-19
修回日期:2017-00-00
作者简介:马倩(1987),女,讲师,主要从事纺织新材料的研究
通信作者:王曙东,博士,sdwang1983@163.com。
更新日期/Last Update: 2017-09-13