|本期目录/Table of Contents|

[1]王海燕,杨金龙,谷山林,等.桑叶槲皮素提取物抗氧化活性研究[J].丝绸,2018,55(3):031103.
 WANG Haiyan,YANG Jinlong,GU Shanlin,et al.Study on antioxidant activity of quercetin extract from mulberry leaves[J].Journal of Silk,2018,55(3):031103.
点击复制

桑叶槲皮素提取物抗氧化活性研究(PDF)
分享到:

《丝绸》[ISSN:1001-7003/CN:33-1122/TS]

卷:
55
期数:
2018年3期
页码:
031103
栏目:
研究与技术
出版日期:
2018-03-20

文章信息/Info

Title:
Study on antioxidant activity of quercetin extract from mulberry leaves
文章编号:
1001-7003(201)00-0000-00
作者:
王海燕杨金龙谷山林王小燕王介平周 婵吕金凤曾秀
重庆市畜牧科学院,重庆 402460
Author(s):
WANG Haiyan YANG Jinlong GU Shanlin WANG Xiaoyan WANG Jieping ZHOU Chan Lü Jinfeng ZENG Xiu
Chongqing Academy of Animal Sciences, Chongqing 402460, China
关键词:
桑叶大孔树脂法槲皮素抗氧化性抗坏血酸
Keywords:
morus leaves macroporous resin method quercetin antioxidant activity ascorbic acid
分类号:
TS195.644
doi:
-
文献标志码:
A
摘要:
桑叶是药食两用植物资源,桑叶槲皮素是其主要的黄酮类化合物,具有广泛生理活性。为了更深入研究桑叶槲皮素活性作用,对桑叶槲皮素抗氧化性展开研究采用大孔树脂法对桑叶槲皮素提取物进行纯化,并对其抗氧化活性进行研究。结果表明:在还原力、清除DPPH方面桑叶槲皮素纯化物较槲皮素粗提取作用较好;在清除超氧阴离子作用方面,低浓度时粗提物优于纯化物,高浓度时纯化物优于粗提物,在清除羟基自由基作用时,粗提物则表现比纯化物较强的清除作用。
Abstract:
The Morus alba L.Leaf is a kind of medicinal and edible plant leaves. Quercetin is the main flavonoids in mulberry leaves, with a wide range of physiological activity. In order to study the activity of Quercetin in mulberry leaves, this paper studies the antioxidant activity of quercetin in mulberry leaves. The quercetin extract from mulberry leaves was purified by macroporous resin method, and its antioxidant activity was studied. The results showed that the crude extract of mulberry leaf quercetin was better than that of quercetin in reducing power and eliminating the DPPH, and the crude extract was superior to the purified product in the removal of superoxide anion; high concentration of the purification was better than the crude extract; in the removal of hydroxyl radicals; the crude extract was better than the purification of the role of purification

参考文献/References:

[1]赖玲林, 彭小芳, 冷恩念. 中药桑叶药理作用的研究进展[J]. 安徽医药, 2016, 20(12): 2210-2214.
LAI Xiaolin, PENG fang, LENG Ennian. Advances in study on pharmacological effects of mulberry leaves [J]. Anhui Medical and Pharmaceutical Journal, 2016, 20(12): 2210-2214.
[2]苏方华. 桑叶的化学成分及临床应用研究进展[J]. 中国医药导报, 2010, 7(14): 9-12.
SU Fanghua. The research progress of the chemical composition and clinical application of mulberry leaves [J]. China Medical Herald, 2010, 7(14): 9-12.
[3]孟庆海, 殷秋忆, 郭静. 4种不同桑叶提取物降血糖作用的筛选[J]. 中成药, 2014, 36(6): 1288-1291.
MENG Qinghai, YING Qiuyi, GUO Jing. Screening of hypoglycemic effects of four different mulberry leaf extracts [J]. Chinese Traditional Patent Medicine, 2014, 36(6): 1288-1291.
[4]吴雯, 梁凯伦, 陈波, 等. 桑叶提取物对食源性肥胖大鼠的减肥作用及机制研究[J].中国中药杂志, 2017, 42(9): 1757-1761.
WU Wen, LIANG Kailun, CHEN Bo, et al. Effects of ori folium extract on diet-induced obesity mechanism in rats [J]. China Journal of Chinese Materia Medica, 2017, 42(9): 1757-1761.
[5]匡荣, 刘璐, 刘艳, 等. 葫芦巴籽和桑叶提取混合物对胰岛素抵抗糖脂代谢紊乱大鼠模型的降血糖作用及其机制研究[J]. 中国现代应用药学, 2016, 33(5): 551-556.
KUANG Rong, LIU Lu, LIU Yan, et al. Anti-hyperglycemic effect of fenugreek seed and mulberry leaf extract formula on the blood glucose levels of the insulin resistance and metabolism disorder rats models and the mechanism [J]. Chinese Journal of Modern Applied Pharmacy, 2016, 33(5): 551-556.
[6]季涛. 桑叶防治糖尿病的效应成分群及其作用机制研究[D]. 南京: 南京中医药大学, 2016.
JI Tao. The Study on Active Components Related to Diabetes in Mulberry Leaves and Its Action Mechanism [D]. Nanjing: Nanjing Univeersty of Chinese Medicine, 2016.
[7]唐明敏, 刘洋, 田思敏, 等. 桑叶不同提取部位对α葡萄糖苷酶抑制活性体外研究[J]. 新疆医科大学学报, 2015,38(6): 711-714.
TANG Mingmin, LIU Yang, TIAN Simin, et al. α-glucosidase inhibition by different extracts of folium mori in vitro assay [J]. Journal of Xinjiang Medical University, 2015, 38(6): 711-714.
[8]邹莉芳, 王玲, 丁晓雯, 等. 桑叶水提物预防小鼠高脂血症的作用研究[J]. 食品安全质量检测学报, 2016, 7(8): 3039-3045.
ZOU Lifang, WANG Ling, DING Xiaowen, et al.Effect study of mulberry leaf water extract prophylaxis of hyperlipemia in high-fat diet mice [J]. Journal of Food Safety &Quality, 2016, 7(8): 3039-3045.
[9]张贵会, 王贺, 杨玲. 新疆药桑叶中黄酮类化合物的分离及其抗氧化活性评价[J]. 中国酿造, 2016, 3(2): 101-105.
ZHANG Guihui, WANG He, YANG Ling. Isolation of flavonoids compounds in morus nigra leaves from Xinjiang and their antioxidant activity [J]. China Brewing, 2016, 35(2): 101-105.
[10]侯瑞宏, 廖森泰, 刘凡, 等. 桑叶多糖对小鼠免疫调节作用的影响[J]. 食品科学, 2011, 32(13): 280-283.
HOU Ruihong, LIAO Sentai, LIU Fan, et al. Immunomodulatory effect of polysaccharides from mulberry leaves (pml) in mice [J]. Food Science, 2011, 32(13): 280-283.
[11]王娅, 李嘉盈. 桑叶抗糖尿病活性成分研究[J]. 安徽医药, 2016, 20(3): 425-428.
WANG Ya, LI Jiaying. Anti-diabetic constituents of mulberry leaf [J]. Anhui Medical and Pharmaceutical Journal, 2016, 20(3): 425-428.
[12]MAHMOOD T, ANWAR F, ABBAS M, et al. Effect of maturity on phe-nolics (phenolic acids and flavonoids) profile of strawberry culti-vars and mulberry species from pakistan [J]. International Journalof Molecular Sciences, 2011, 13(4): 4591-4607.
[13]AOKI F, HONDA S,KISHIDA H, et al. Suppression by licorice fla-vonoids of abdominal fat accumulation and body weight gain inhigh-fat diet-induced obese C57BL/6J mice [J]. Bioscience Bio-technology & Biochemistry, 2007, 71(1): 206-214.
[14]SUN X F, YAMASAKI M, KATSUBE T, et al. Effects of quercetin derivatives from mulberry leaves: improved gene expression related he-patic lipid and glucose metabolism in short-term high-fat fed mice [J]. Nutrition Research & Practice, 2015, 9(2): 137-143.
[15]ZHENG S, LIAO S, ZOU Y, et al. Mulberry leaf polyphenols delay ag-ing and regulate fat metabolism via the germline signaling pathwayin caenorhabditis elegans [J]. Journal of the American Aging As-sociation, 2014, 36( 6): 1-13.
[16]顾远晖, 李一平, 李安强, 等. 槲皮素对人肝癌SMMC-7721细胞生长的抑制及细胞凋亡的诱导作用[J]. 兰州大学学报(医学版), 2010(3) : 9.
GU Yuanhui, LI Yiping, LI Anqiang, et al. Mechanisms of growth inhibition and apoptosis inducement of quercetin on human hepatocellular carcinoma SMMC-7721 cells [J]. Journal of Lanzhou University (Medical Sciences), 2010,36(3): 9.
[17]张丹丹, 陈娟娟, 张亚伟, 等. 连翘及其主要有效成分槲皮素体外抗人巨细胞病毒的实验研究[J]. 中国中药杂志, 2010, 35(8): 1055.
ZHAGN Dandan, CHEN Juanjuan, ZHANG Yawei, et al. An invitro study of anti-human cy egalovirus effect of forsythia suspense and its main active ingredient quercetin [J]. China Journal of Chinese Materia Medica, 2010, 35(8): 1055.
[18]尹军强, 谢显彪, 贾强, 等. 槲皮素对人骨肉瘤细胞 U-2OS/MTX300 增殖和凋亡的影响及其机制研究[J]. 中国中药杂志, 2012, 37(5): 611.
YIN Junqiang, XIE Xianbiao, JIA Qiang, et al. Effect and mechanism of quercetin on proliferation and apoptosis of human osteosarcoma cell U-2OS / MTX300 [J]. China Journal of Chinese Materia Medica, 2012, 37(5): 611.
[19]DESGROSELLIER J S, CHERESH D A. Integrins in cancer: biological implications and therapeutic opportunities [J]. Nat Rev Cancer, 2010, 10(1): 9.
[20]戴开金, 罗奇志, 侯连兵. HPLC法测定桑叶中芦丁、绿原酸和槲皮素[J]. 南方医科大学学报, 2009, 29(3): 579-580.
DAI Kaijin, LUO Qizhi, HOU Lianbing. Determination of rutin, chlorogenic acid and quercetin in mulberry leaves by HPLC [J]. Journal of Southern Medical University, 2009, 29(3):579-580.
[21]于小蓉, 孙莲, 孟磊. HPLC测定新疆药桑叶中绿原酸、芦丁、异槲皮苷和槲皮素[J].中国现代应用药学, 2011, 28(11): 1051-1054.
YU Xiaorong, SUN Lian, MENG Lei. Determination of chlorogenic acid, rutin, isoquercitrin and quercetin in drug mulberry leaves of Xinjiang by HPLC [J]. Chinese Journal of Modern Applied Pharmacy, 2011, 28(11): 1051-1054.
[22]王海燕, 李睿, 曾秀, 等. 响应面优化超声波提取桑叶槲皮素工艺[J]. 食品科学, 2014, 35(22): 56-62.
WANG Haiyan, LI Rui, ZENG Xiu, et al. Optimization of ultrasound-assisted extraction of quercetin from mulberry leaves by response surface methodology [J]. Food Science, 2014, 35(22): 56-62.
[23]王俊, 赵辉, 吴福安. 树脂吸附法分离纯化桑叶总黄酮(I) H103 树脂对桑叶水提液中总黄酮的吸附性能[J]. 离子交换与吸附, 2008, 24(2): 139-147.
WANG Jun, ZHAO Hui, WU Fuan. Separation and purification of total flavones from mulberry leaves (I) the characters of adsorbability of H103 resin to total flavones [J]. Ion Exchange and Adsorption, 2008, 24(2): 139-147.
[24]徐艳阳, 李科静, 贾洪雷. 荧光法测定桑叶中槲皮素含量的优化[J]. 现代食品科技, 2013, 29(11):2706-2711.
XU Yanyang, LI Kejing, JIA Honglei. Method optimization of determining quercetin content in mulberryleaf by fluorescence spectrophotometry [J]. Modern Food Science and Technology, 2013, 29(11):2706-2711.
[25]蒋立娣, 宣贵达, 吴好好. 桑叶提取物中槲皮素和山萘酚的含量测定[J]. 浙江大学学报(理学版), 2009, 36(6): 706-707.
JIANG Lidi, XUAN Guida, WU Haohao, et al. Determination of quercetin and kaempferol in folium mori extract after hydrolysis by hydrochloric acid [J]. Journal of Zhejiang University (Science Edition), 2009, 36(6): 705-707.
[26]牛天羽. 4种桑的桑葚与桑叶有效成分和生物活性研究[D]. 吉林: 吉林大学, 2015:48-49.
NIU Tianyu. Research on onstituents and Bioactivities of Four Kinds Mulberry Fruits and Mulberry Leaves [D]. Jilin: Jilin University, 2015: 48-49.
[27]陈柳萌. 半枝莲、白花蛇舌草药对提取浸膏中抗氧化活性成分研究[D]. 南昌: 南昌大学, 2010: 32.

备注/Memo

备注/Memo:
基金项目: 重庆市基础科研应用开发计划项目(16439);重庆市农发资金项目(15403)
收稿日期:2017-07-24
修回日期:2017-00-00
作者简介:王海燕(1986—),女,助理研究员,硕士,主要从事天然活性成分、蚕桑资源综合利用及食品安全与质量控制的研究
通信作者:杨金龙,研究员, whydetian@163.com
更新日期/Last Update: 2018-01-15