|本期目录/Table of Contents|

[1]魏如男,王富平,张 媚,等.丝素肽络合Ca2+含量影响细胞生长的研究[J].丝绸,2019,56(3):031102.[doi:10.3969/j.issn.1001-7003.2019.03.002]
 WEI Runan,WANG Fuping,ZHANG Mei,et al.Study on the effect of silk fibroin peptide complex Ca2+ content on cell growth[J].Journal of Silk,2019,56(3):031102.[doi:10.3969/j.issn.1001-7003.2019.03.002]





Study on the effect of silk fibroin peptide complex Ca2+ content on cell growth
魏如男王富平张 媚陈忠敏
重庆理工大学 药学与生物工程学院,重庆 400054
WEI Runan WANG Fuping ZHANG Mei CHEN Zhongmin
College of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China
silk fibroin peptide Ca2+ content cell growth relative molecular mass secondary structure
研究丝素肽(SFP)络合Ca2+含量对人正常肝细胞L02生长的影响。采用CaCl2-乙醇-水三元体系制备(SF),并获得不同Ca2+含量的SFP后进行相对分子质量分级;采用EDTA滴定法测定SFP中Ca2+含量,细胞增殖率评价其细胞毒性,圆二色谱(CD)检测二级结构。结果表明:随透析时间(12、24、36 h)增加,SFP中Ca2+含量分别为16.00%、9.60%、0.40%,逐渐减小,对L02的生长作用由促进转变为抑制,其中在SFP相对分子质量大于8 kD时尤为明显,L02细胞增殖率从118.68%±3.65%)减小到59.78%±1.70%),对应SFP二级结构中3.43%的α螺旋和5.35%的无规则卷曲转变为β折叠。当SFP应用于组织工程支架领域时,络合较高Ca2+含量的SFP更有利于细胞的增殖。
The effects of silk fibroin peptide (SFP) complex Ca2+ content on the growth of human normal hepatocyte L02 were studied. The CaCl2-ethanol-water system was used to prepare silk fibroin (SF), and SFP with different Ca2+ content was obtained. Then, the relative molecular mass of SFP was classified. The content of Ca2+ in SFP was measured by EDTA titration. The cytotoxicity of L02 cells was evaluated by the proliferation rate, and circular dichroism (CD) was used to detect the secondary structure of SFP. The results show that as the time of dialysis (12, 24, 36h) increased, Ca2+ content of SFP (16.00%, 9.60%, 0.40%) gradually decreased, and the growth of L02 changed from promotion to inhibition. Especially when the relative molecular mass of SFP was greater than 8kD, the cell proliferation rate of L02 decreased from (118.68%±3.65%) to (59.78%±1.70%). In the secondary structure of the corresponding SFP, 3.43% of alpha-helix and 5.35% of irregular curl transformed to beta-folding. In the field of tissue engineering scaffolds, SFP with higher content of complex Ca2+ is more conducive to cell proliferation


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更新日期/Last Update: 2019-01-18